Illumina 測序組合試劑盒

Illumina 測序組合試劑盒

"Illumina/TruSight HLA v2 Sequencing Panel (24 samples)/20000215/1 Ea

" 20000215 Illumina 產品編號： 20000215美  元  價： $0.00會  員  價： 待定品       牌： Illumina產       地： 美國公       司： Illumina, Inc.產品分類： 其它>其它試劑>芯片公司分類： Clinical Research Products "Product Highlights:

The TruSight HLA v2 Sequencing Panel provides clinical researchers with a broad-coverage, ultra-high–resolution human leukocyte antigen (HLA) typing solution for simple, rapid assessment of the HLA region in a single assay. It features:

High-resolution sequencing of 11 HLA loci

Workflow with library preparation, sequencing and data analysis/reporting

Accurate and high-resolution HLA typing for unambiguous results

Capture Full HLA Gene Sequences

TruSight HLA v2 covers all commonly typed HLA loci, plus those with emerging relevance. This panel enables sequencing of Class I HLA-A, B, and C; Class II HLA-DRB1/3/4/5, HLA-DQA1, HLA-DQB1, HLA-DPA1, and HLA-DPB1. This expands coverage beyond the classic loci, with full gene coverage, enabling discovery of new alleles without the need to design new primers.

Sample-to-Report Workflow with Optimized Data Analysis

The comprehensive solution includes reagents and software optimized for HLA analysis. A simplified workflow enables turnaround time of less than 48 hours and increased efficiency with less than 4.5 hours of hands-on time. On-instrument software analyzes sequence data; TruSight HLA Assign Software provides flexIBLe options for post-analysis reporting of results.

High-Accuracy HLA Typing

The expanded coverage of TruSight HLA v2 provides high resolution, eliminating the need for follow-up testing to obtain a confident typing result. View HLA Loci.

How TruSight HLA v2 Works

TruSight HLA v2 amplifies HLA loci with long-range PCR. After amplification, Nextera tagmentation fragments the DNA and leaves behind a sequence tag. An additional PCR then adds sequence adapters and indexing primers to generate sequencing-ready DNA libraries. Prepared libraries are then loaded directly onto a MiniSeq, MiSeq or NextSeq System for sequencing.

Analysis Software Highlights

The Assign 2.0 TruSight HLA analysis software assists with the assignment of a human leukocyte antigen (HLA) type. The software is designed to analyze data from libraries prepared with Illumina TruSight HLA Sequencing Panels and then sequenced on an Illumina sequencer. Use Assign 2.0 to import sequence data, perform base calling, edit sequences, and compare a sample consensus sequence with the IMGT/HLA database of alleles.

Specifications:

Assay Time 48 hours

Hands-On Time 4.5 hours

Input Quantity 400 ng DNA

System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500

Variant Class Single Nucleotide Polymorphisms (SNPs)

Technology Sequencing

Species Category Human

Method HLA Sequencing"

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